Caloosahatchee Creeks Preserve is located on the north bank of the Caloosahatchee River. The preserve is separated by Interstate 75 and includes two separate access points, one on the east side of the interstate and another on the west side.

On the east side, visitors can enjoy a 1.5-mile boardwalk and paved trail that meanders through wetland, hammock, and pine flatwood ecosystems, as well as three miles of additional primitive hiking trails.

On the west side, visitors can access a canoe/kayak launch into Popash Creek, which eventually flows into the Caloosahatchee River. Additionally, visitors can enjoy a short hiking trail and boardwalk that includes an observation deck. A Lee County Parks & Recreation parking fee of $1/hour or $5/day applies only on the west side.

Both sides include paved parking, picnic pavilions and a restroom. On-leash pet-walking is also permitted on both sides of this preserve.